Composite
Part:BBa_K5477025:Design
Designed by: Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
pRET2 - ARNT receptor module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 810
Illegal EcoRI site found at 1916
Illegal SpeI site found at 1760 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 810
Illegal EcoRI site found at 1916
Illegal NheI site found at 182
Illegal SpeI site found at 1760 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 810
Illegal EcoRI site found at 1916 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 810
Illegal EcoRI site found at 1916
Illegal SpeI site found at 1760 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 810
Illegal EcoRI site found at 1916
Illegal SpeI site found at 1760
Illegal AgeI site found at 37 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This module supports the AhR-mediated response to toxic ligands like PAHs, dioxins and PCBs by binding to the XRE() in our reporter module facilitating the transcription of NanoLuc (). The composite part was cloned using the method of USER-cloning into YCp-H. YCp-H is a centromeric plasmid used in yeast that includes a HIS3 marker, allowing for selection in histidine auxotrophic yeast strains. Like other CEN plasmids, YCp-H contains a CEN sequence, ensuring that the plasmid replicates and segregates similarly to yeast chromosomes. This results in a low copy number (typically one to two copies per cell), providing stable maintenance of the plasmid.
Source
Homo sapiens and Saccharomyces cerevisiae